Review



rabbit anti p stat3 tyr705  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc rabbit anti p stat3 tyr705
    Rabbit Anti P Stat3 Tyr705, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+p+stat3+tyr705/pmc12999353-6-0-4
    Average 86 stars, based on 1 article reviews
    rabbit anti p stat3 tyr705 - by Bioz Stars, 2026-09
    86/100 stars

    Images

    Related Articles

    Membrane:

    Article Title: Genistein-3'-sodium sulfonate ameliorates cerebral ischemia injuries by blocking neuroinflammation through the α7nAChR-JAK2/STAT3 signaling pathway in rats.
    Article Snippet: After gel electrophoresis (Mini-protean, Bio-Rad, Hercules, CA), a slab sliver was excised, and the protein was transferred to a PVDF membrane (Millipore, CA). .. Then, the membrane was further blocked for 60 min by non-fat blocking-grade milk (5%) in Tris-buffered saline with Tween-20 (0.1%), followed by incubation at 4 ◦C overnight with primary antibody of rabbit anti-α7nAChR (1:200, ab10096, Abcam, UK), rabbit anti-IL-6 (1:200, SC-1265, Santa Cruz, CA), rabbit anti-IL-1β (1:200, ab6722, Abcam, UK), rabbit anti-TNF-α (1:800, Ab66579, Abcam, UK), mouse anti-JAK2 (1:800, E4Y4D, Cell Signaling Technology, USA), rabbit anti-p-JAK2 (1:200,TYR1007, Cell Signaling Technology, USA), mouse anti-STAT3 (1:800, 124H6, Cell Signaling Technology, USA), rabbit anti-p-STAT3 (Tyr705) (1:200, 9145S, Cell Signaling Technology, USA), or rabbit anti-β-actin (1:1000, Solarbio, China). .. Afterwards, blots were washed followed by incubation in the presence of horseradish peroxidase-conjugated anti-rabbit secondary antibody (1:1000, Cell Signaling Technology, USA) at 4 ◦C for 4 h or overnight.

    Blocking Assay:

    Article Title: Genistein-3'-sodium sulfonate ameliorates cerebral ischemia injuries by blocking neuroinflammation through the α7nAChR-JAK2/STAT3 signaling pathway in rats.
    Article Snippet: After gel electrophoresis (Mini-protean, Bio-Rad, Hercules, CA), a slab sliver was excised, and the protein was transferred to a PVDF membrane (Millipore, CA). .. Then, the membrane was further blocked for 60 min by non-fat blocking-grade milk (5%) in Tris-buffered saline with Tween-20 (0.1%), followed by incubation at 4 ◦C overnight with primary antibody of rabbit anti-α7nAChR (1:200, ab10096, Abcam, UK), rabbit anti-IL-6 (1:200, SC-1265, Santa Cruz, CA), rabbit anti-IL-1β (1:200, ab6722, Abcam, UK), rabbit anti-TNF-α (1:800, Ab66579, Abcam, UK), mouse anti-JAK2 (1:800, E4Y4D, Cell Signaling Technology, USA), rabbit anti-p-JAK2 (1:200,TYR1007, Cell Signaling Technology, USA), mouse anti-STAT3 (1:800, 124H6, Cell Signaling Technology, USA), rabbit anti-p-STAT3 (Tyr705) (1:200, 9145S, Cell Signaling Technology, USA), or rabbit anti-β-actin (1:1000, Solarbio, China). .. Afterwards, blots were washed followed by incubation in the presence of horseradish peroxidase-conjugated anti-rabbit secondary antibody (1:1000, Cell Signaling Technology, USA) at 4 ◦C for 4 h or overnight.

    Article Title: Dimethyl fumarate improves cognitive impairment and neuroinflammation in mice with Alzheimer's disease.
    Article Snippet: .. Twenty-micrometer-thick coronal brain sections were fixed with 4% paraformaldehyde in PBS for 5 min, and then we performed antigen retrieval with HistoVT One (Nakarai, Kyoto, JAPAN) 70 °C for 20 min. After incubation in blocking solution (5% goat or donkey serum/PBS) for 1 h, sections were incubated with a combination of the following antibodies: rabbit anti-Iba-1 (#019–19741, 1:500; FUJIFILM Wako Osaka, Japan), goat anti-AIF-1/Iba1 (#NB1001028, 1:250; Novus Biologicals, CO, USA), mouse anti-Aβ (#10326, 1:200; IBL, Nagoya, Japan), mouse antiGFAP (#G3893, 1:250, Sigma), rat anti-C3 (#sc58926, 1:200; Santa Cruz Biotechnology, Inc., CA, USA), rabbit anti-BACE1 (#5606, 1:100, Cell Signaling, Inc., CA, USA), and rabbit anti-p-STAT3 (Tyr705) (#9145, 1:2000, Cell Signaling Technology, Inc.) at 4 °C overnight. .. After washing with PBS, the sections were incubated with fluorescent-conjugated anti-rabbit, anti-mouse, anti-goat, or anti-rat IgG (1:1000; Thermo Fisher Scientific) and 4ʹ,6ʹ-diamidino-2-phenylindole (DAPI) (1:2000) at room temperature for 1 h. After washing, the sections were mounted on slides with Fluoromount/PlusTM (Diagnostic BioSystems, Pleasanton, CA, USA) and analyzed using a confocal microscope (LSM700, Carl Zeiss, Oberkochen, Germany).

    Saline:

    Article Title: Genistein-3'-sodium sulfonate ameliorates cerebral ischemia injuries by blocking neuroinflammation through the α7nAChR-JAK2/STAT3 signaling pathway in rats.
    Article Snippet: After gel electrophoresis (Mini-protean, Bio-Rad, Hercules, CA), a slab sliver was excised, and the protein was transferred to a PVDF membrane (Millipore, CA). .. Then, the membrane was further blocked for 60 min by non-fat blocking-grade milk (5%) in Tris-buffered saline with Tween-20 (0.1%), followed by incubation at 4 ◦C overnight with primary antibody of rabbit anti-α7nAChR (1:200, ab10096, Abcam, UK), rabbit anti-IL-6 (1:200, SC-1265, Santa Cruz, CA), rabbit anti-IL-1β (1:200, ab6722, Abcam, UK), rabbit anti-TNF-α (1:800, Ab66579, Abcam, UK), mouse anti-JAK2 (1:800, E4Y4D, Cell Signaling Technology, USA), rabbit anti-p-JAK2 (1:200,TYR1007, Cell Signaling Technology, USA), mouse anti-STAT3 (1:800, 124H6, Cell Signaling Technology, USA), rabbit anti-p-STAT3 (Tyr705) (1:200, 9145S, Cell Signaling Technology, USA), or rabbit anti-β-actin (1:1000, Solarbio, China). .. Afterwards, blots were washed followed by incubation in the presence of horseradish peroxidase-conjugated anti-rabbit secondary antibody (1:1000, Cell Signaling Technology, USA) at 4 ◦C for 4 h or overnight.

    Incubation:

    Article Title: Genistein-3'-sodium sulfonate ameliorates cerebral ischemia injuries by blocking neuroinflammation through the α7nAChR-JAK2/STAT3 signaling pathway in rats.
    Article Snippet: After gel electrophoresis (Mini-protean, Bio-Rad, Hercules, CA), a slab sliver was excised, and the protein was transferred to a PVDF membrane (Millipore, CA). .. Then, the membrane was further blocked for 60 min by non-fat blocking-grade milk (5%) in Tris-buffered saline with Tween-20 (0.1%), followed by incubation at 4 ◦C overnight with primary antibody of rabbit anti-α7nAChR (1:200, ab10096, Abcam, UK), rabbit anti-IL-6 (1:200, SC-1265, Santa Cruz, CA), rabbit anti-IL-1β (1:200, ab6722, Abcam, UK), rabbit anti-TNF-α (1:800, Ab66579, Abcam, UK), mouse anti-JAK2 (1:800, E4Y4D, Cell Signaling Technology, USA), rabbit anti-p-JAK2 (1:200,TYR1007, Cell Signaling Technology, USA), mouse anti-STAT3 (1:800, 124H6, Cell Signaling Technology, USA), rabbit anti-p-STAT3 (Tyr705) (1:200, 9145S, Cell Signaling Technology, USA), or rabbit anti-β-actin (1:1000, Solarbio, China). .. Afterwards, blots were washed followed by incubation in the presence of horseradish peroxidase-conjugated anti-rabbit secondary antibody (1:1000, Cell Signaling Technology, USA) at 4 ◦C for 4 h or overnight.

    Article Title: Dimethyl fumarate improves cognitive impairment and neuroinflammation in mice with Alzheimer's disease.
    Article Snippet: .. Twenty-micrometer-thick coronal brain sections were fixed with 4% paraformaldehyde in PBS for 5 min, and then we performed antigen retrieval with HistoVT One (Nakarai, Kyoto, JAPAN) 70 °C for 20 min. After incubation in blocking solution (5% goat or donkey serum/PBS) for 1 h, sections were incubated with a combination of the following antibodies: rabbit anti-Iba-1 (#019–19741, 1:500; FUJIFILM Wako Osaka, Japan), goat anti-AIF-1/Iba1 (#NB1001028, 1:250; Novus Biologicals, CO, USA), mouse anti-Aβ (#10326, 1:200; IBL, Nagoya, Japan), mouse antiGFAP (#G3893, 1:250, Sigma), rat anti-C3 (#sc58926, 1:200; Santa Cruz Biotechnology, Inc., CA, USA), rabbit anti-BACE1 (#5606, 1:100, Cell Signaling, Inc., CA, USA), and rabbit anti-p-STAT3 (Tyr705) (#9145, 1:2000, Cell Signaling Technology, Inc.) at 4 °C overnight. .. After washing with PBS, the sections were incubated with fluorescent-conjugated anti-rabbit, anti-mouse, anti-goat, or anti-rat IgG (1:1000; Thermo Fisher Scientific) and 4ʹ,6ʹ-diamidino-2-phenylindole (DAPI) (1:2000) at room temperature for 1 h. After washing, the sections were mounted on slides with Fluoromount/PlusTM (Diagnostic BioSystems, Pleasanton, CA, USA) and analyzed using a confocal microscope (LSM700, Carl Zeiss, Oberkochen, Germany).



    Similar Products

    86
    Cell Signaling Technology Inc rabbit anti p stat3 tyr705
    Rabbit Anti P Stat3 Tyr705, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+p+stat3+tyr705/pmc12999353-6-0-4
    Average 86 stars, based on 1 article reviews
    rabbit anti p stat3 tyr705 - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    98
    Cell Signaling Technology Inc anti phosphorylated stat3 p stat3
    Anti Phosphorylated Stat3 P Stat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+p+stat3+tyr705/Phospho-Stat3+(Tyr705)+XP+Rabbit+mAb/pm41928364-99-27-31
    Average 98 stars, based on 1 article reviews
    anti phosphorylated stat3 p stat3 - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    98
    Cell Signaling Technology Inc anti p stat3
    Anti P Stat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+p+stat3+tyr705/Phospho-Stat3+(Tyr705)+XP+Rabbit+mAb/pmc12917872-76-46-48
    Average 98 stars, based on 1 article reviews
    anti p stat3 - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    98
    Cell Signaling Technology Inc p stat3
    P Stat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+p+stat3+tyr705/Phospho-Stat3+(Tyr705)+XP+Rabbit+mAb/pm41917326-357-52-53
    Average 98 stars, based on 1 article reviews
    p stat3 - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    98
    Cell Signaling Technology Inc p stat3 tyr705
    P Stat3 Tyr705, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+p+stat3+tyr705/Phospho-Stat3+(Tyr705)+XP+Rabbit+mAb/pm41900081-186-57-59
    Average 98 stars, based on 1 article reviews
    p stat3 tyr705 - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    98
    Cell Signaling Technology Inc phosphorylated stat3 tyr705 p stat3
    KSM inhibits CHI3L1-stimulated M2 macrophage activation via EGFR. Bulk RNA-seq sequencing analysis on the differentiated macrophages from THP-1 cells was used to evaluate mRNA expression in the macrophage differentiation regulated by CHI3L1 and KSM. ( A ). Volcano plots showing differentially expressed genes regulated by CHI3L1. ( B ) Representative plots of the top 20 genes that are upregulated (> 2-fold) by CHI3L1 stimulation but downregulated (< 2-fold) by KSM treatment. ( C ) Representative mRNA expression of EGFR in differentiated macrophages with CHI3L1 and KSM treatment was detected by real-time qRT-PCR. ( D ) Representative immunoblots showing p-EGFR (Tyr1068) and total EGFR expression in differentiated macrophages with CHI3L1 and KSM. Right panel, densitometric quantitation on the blots of EGFR and p-EGFR. ( E ) Effect of EGFR inhibition on the expression of <t>p-STAT3</t> and total-STAT3 in CHI3L1-stimulated, differentiated macrophages by gefitinib (Tocris Bioscience, #3000) treatment (1 μM, 72 hours). Right panel, densitometric quantitation on the blots of p-STAT3 and total STAT3. ( F ) Effects of EGFR inhibition on the expression of CD163 and CD206 in CHI3L1-stimulated, differentiated macrophages by gefitinib treatment (0.1 and 1 μM, 72 hours). Right panel, densitometric quantitation on the blots of CD163 and CD206. The values in ( C – F ) are the mean ± SEM. * P <0.05, ** P <0.01, *** P <0.001 (One-Way ANOVA, multiple comparisons).
    Phosphorylated Stat3 Tyr705 P Stat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+p+stat3+tyr705/Phospho-Stat3+(Tyr705)+XP+Rabbit+mAb/pmc13007551-66-76-82
    Average 98 stars, based on 1 article reviews
    phosphorylated stat3 tyr705 p stat3 - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    Image Search Results


    KSM inhibits CHI3L1-stimulated M2 macrophage activation via EGFR. Bulk RNA-seq sequencing analysis on the differentiated macrophages from THP-1 cells was used to evaluate mRNA expression in the macrophage differentiation regulated by CHI3L1 and KSM. ( A ). Volcano plots showing differentially expressed genes regulated by CHI3L1. ( B ) Representative plots of the top 20 genes that are upregulated (> 2-fold) by CHI3L1 stimulation but downregulated (< 2-fold) by KSM treatment. ( C ) Representative mRNA expression of EGFR in differentiated macrophages with CHI3L1 and KSM treatment was detected by real-time qRT-PCR. ( D ) Representative immunoblots showing p-EGFR (Tyr1068) and total EGFR expression in differentiated macrophages with CHI3L1 and KSM. Right panel, densitometric quantitation on the blots of EGFR and p-EGFR. ( E ) Effect of EGFR inhibition on the expression of p-STAT3 and total-STAT3 in CHI3L1-stimulated, differentiated macrophages by gefitinib (Tocris Bioscience, #3000) treatment (1 μM, 72 hours). Right panel, densitometric quantitation on the blots of p-STAT3 and total STAT3. ( F ) Effects of EGFR inhibition on the expression of CD163 and CD206 in CHI3L1-stimulated, differentiated macrophages by gefitinib treatment (0.1 and 1 μM, 72 hours). Right panel, densitometric quantitation on the blots of CD163 and CD206. The values in ( C – F ) are the mean ± SEM. * P <0.05, ** P <0.01, *** P <0.001 (One-Way ANOVA, multiple comparisons).

    Journal: ImmunoTargets and Therapy

    Article Title: Kasugamycin Inhibits Melanoma Lung Metastasis and CHI3L1-Driven M2-Like Tumor-Associated Macrophage Differentiation

    doi: 10.2147/ITT.S563951

    Figure Lengend Snippet: KSM inhibits CHI3L1-stimulated M2 macrophage activation via EGFR. Bulk RNA-seq sequencing analysis on the differentiated macrophages from THP-1 cells was used to evaluate mRNA expression in the macrophage differentiation regulated by CHI3L1 and KSM. ( A ). Volcano plots showing differentially expressed genes regulated by CHI3L1. ( B ) Representative plots of the top 20 genes that are upregulated (> 2-fold) by CHI3L1 stimulation but downregulated (< 2-fold) by KSM treatment. ( C ) Representative mRNA expression of EGFR in differentiated macrophages with CHI3L1 and KSM treatment was detected by real-time qRT-PCR. ( D ) Representative immunoblots showing p-EGFR (Tyr1068) and total EGFR expression in differentiated macrophages with CHI3L1 and KSM. Right panel, densitometric quantitation on the blots of EGFR and p-EGFR. ( E ) Effect of EGFR inhibition on the expression of p-STAT3 and total-STAT3 in CHI3L1-stimulated, differentiated macrophages by gefitinib (Tocris Bioscience, #3000) treatment (1 μM, 72 hours). Right panel, densitometric quantitation on the blots of p-STAT3 and total STAT3. ( F ) Effects of EGFR inhibition on the expression of CD163 and CD206 in CHI3L1-stimulated, differentiated macrophages by gefitinib treatment (0.1 and 1 μM, 72 hours). Right panel, densitometric quantitation on the blots of CD163 and CD206. The values in ( C – F ) are the mean ± SEM. * P <0.05, ** P <0.01, *** P <0.001 (One-Way ANOVA, multiple comparisons).

    Article Snippet: Protein lysates (25 μg) from lung tissues or cells were subjected to SDS-PAGE, transferred to membranes, and immunoblotted with primary antibodies against CD163 (#PA5-109327, Thermo Fisher Scientific), CD206 (E6T5J, #24595S, Cell Signaling Technology), phosphorylated AKT (p-Akt) (193H12, #4058S, Cell Signaling Technology), and total Akt (11E7, #4685S, Cell Signaling Technology), phosphorylated EGFR (Tyr1068) (p-EGFR) (#44-788G, Thermo Fisher Scientific), EGFR (D38B1, #4267S, Cell Signaling Technology), phosphorylated Erk (p-Erk) (#9101S, Cell Signaling Technology), total Erk (#9102S, Cell Signaling Technology), phosphorylated STAT3 (Tyr705) (p-STAT3) (D3A7, #9145, Cell Signaling Technology), total STAT3 (79D7, #4904, Cell Signaling Technology) and b-actin (C4, #sc-47778 HRP, Santa Cruz Biotechnology).

    Techniques: Activation Assay, RNA Sequencing, Sequencing, Expressing, Quantitative RT-PCR, Western Blot, Quantitation Assay, Inhibition