rabbit anti p stat3 tyr705 (Cell Signaling Technology Inc)
Structured Review
Rabbit Anti P Stat3 Tyr705, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+p+stat3+tyr705/pmc12999353-6-0-4
Average 86 stars, based on 1 article reviews
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Membrane:Article Title: Genistein-3'-sodium sulfonate ameliorates cerebral ischemia injuries by blocking neuroinflammation through the α7nAChR-JAK2/STAT3 signaling pathway in rats. Article Snippet: After gel electrophoresis (Mini-protean, Bio-Rad, Hercules, CA), a slab sliver was excised, and the protein was transferred to a PVDF membrane (Millipore, CA). .. Then, the membrane was further blocked for 60 min by non-fat blocking-grade milk (5%) in Tris-buffered saline with Tween-20 (0.1%), followed by incubation at 4 ◦C overnight with primary antibody of rabbit anti-α7nAChR (1:200, ab10096, Abcam, UK), rabbit anti-IL-6 (1:200, SC-1265, Santa Cruz, CA), rabbit anti-IL-1β (1:200, ab6722, Abcam, UK), rabbit anti-TNF-α (1:800, Ab66579, Abcam, UK), mouse anti-JAK2 (1:800, E4Y4D, Cell Signaling Technology, USA), rabbit anti-p-JAK2 (1:200,TYR1007, Cell Signaling Technology, USA), mouse anti-STAT3 (1:800, 124H6, Cell Signaling Technology, USA), Blocking Assay:Article Title: Genistein-3'-sodium sulfonate ameliorates cerebral ischemia injuries by blocking neuroinflammation through the α7nAChR-JAK2/STAT3 signaling pathway in rats. Article Snippet: After gel electrophoresis (Mini-protean, Bio-Rad, Hercules, CA), a slab sliver was excised, and the protein was transferred to a PVDF membrane (Millipore, CA). .. Then, the membrane was further blocked for 60 min by non-fat blocking-grade milk (5%) in Tris-buffered saline with Tween-20 (0.1%), followed by incubation at 4 ◦C overnight with primary antibody of rabbit anti-α7nAChR (1:200, ab10096, Abcam, UK), rabbit anti-IL-6 (1:200, SC-1265, Santa Cruz, CA), rabbit anti-IL-1β (1:200, ab6722, Abcam, UK), rabbit anti-TNF-α (1:800, Ab66579, Abcam, UK), mouse anti-JAK2 (1:800, E4Y4D, Cell Signaling Technology, USA), rabbit anti-p-JAK2 (1:200,TYR1007, Cell Signaling Technology, USA), mouse anti-STAT3 (1:800, 124H6, Cell Signaling Technology, USA), Article Title: Dimethyl fumarate improves cognitive impairment and neuroinflammation in mice with Alzheimer's disease. Article Snippet: .. Twenty-micrometer-thick coronal brain sections were fixed with 4% paraformaldehyde in PBS for 5 min, and then we performed antigen retrieval with HistoVT One (Nakarai, Kyoto, JAPAN) 70 °C for 20 min. After incubation in blocking solution (5% goat or donkey serum/PBS) for 1 h, sections were incubated with a combination of the following antibodies: rabbit anti-Iba-1 (#019–19741, 1:500; FUJIFILM Wako Osaka, Japan), goat anti-AIF-1/Iba1 (#NB1001028, 1:250; Novus Biologicals, CO, USA), mouse anti-Aβ (#10326, 1:200; IBL, Nagoya, Japan), mouse antiGFAP (#G3893, 1:250, Sigma), rat anti-C3 (#sc58926, 1:200; Santa Cruz Biotechnology, Inc., CA, USA), rabbit anti-BACE1 (#5606, 1:100, Cell Signaling, Inc., CA, USA), and Saline:Article Title: Genistein-3'-sodium sulfonate ameliorates cerebral ischemia injuries by blocking neuroinflammation through the α7nAChR-JAK2/STAT3 signaling pathway in rats. Article Snippet: After gel electrophoresis (Mini-protean, Bio-Rad, Hercules, CA), a slab sliver was excised, and the protein was transferred to a PVDF membrane (Millipore, CA). .. Then, the membrane was further blocked for 60 min by non-fat blocking-grade milk (5%) in Tris-buffered saline with Tween-20 (0.1%), followed by incubation at 4 ◦C overnight with primary antibody of rabbit anti-α7nAChR (1:200, ab10096, Abcam, UK), rabbit anti-IL-6 (1:200, SC-1265, Santa Cruz, CA), rabbit anti-IL-1β (1:200, ab6722, Abcam, UK), rabbit anti-TNF-α (1:800, Ab66579, Abcam, UK), mouse anti-JAK2 (1:800, E4Y4D, Cell Signaling Technology, USA), rabbit anti-p-JAK2 (1:200,TYR1007, Cell Signaling Technology, USA), mouse anti-STAT3 (1:800, 124H6, Cell Signaling Technology, USA), Incubation:Article Title: Genistein-3'-sodium sulfonate ameliorates cerebral ischemia injuries by blocking neuroinflammation through the α7nAChR-JAK2/STAT3 signaling pathway in rats. Article Snippet: After gel electrophoresis (Mini-protean, Bio-Rad, Hercules, CA), a slab sliver was excised, and the protein was transferred to a PVDF membrane (Millipore, CA). .. Then, the membrane was further blocked for 60 min by non-fat blocking-grade milk (5%) in Tris-buffered saline with Tween-20 (0.1%), followed by incubation at 4 ◦C overnight with primary antibody of rabbit anti-α7nAChR (1:200, ab10096, Abcam, UK), rabbit anti-IL-6 (1:200, SC-1265, Santa Cruz, CA), rabbit anti-IL-1β (1:200, ab6722, Abcam, UK), rabbit anti-TNF-α (1:800, Ab66579, Abcam, UK), mouse anti-JAK2 (1:800, E4Y4D, Cell Signaling Technology, USA), rabbit anti-p-JAK2 (1:200,TYR1007, Cell Signaling Technology, USA), mouse anti-STAT3 (1:800, 124H6, Cell Signaling Technology, USA), Article Title: Dimethyl fumarate improves cognitive impairment and neuroinflammation in mice with Alzheimer's disease. Article Snippet: .. Twenty-micrometer-thick coronal brain sections were fixed with 4% paraformaldehyde in PBS for 5 min, and then we performed antigen retrieval with HistoVT One (Nakarai, Kyoto, JAPAN) 70 °C for 20 min. After incubation in blocking solution (5% goat or donkey serum/PBS) for 1 h, sections were incubated with a combination of the following antibodies: rabbit anti-Iba-1 (#019–19741, 1:500; FUJIFILM Wako Osaka, Japan), goat anti-AIF-1/Iba1 (#NB1001028, 1:250; Novus Biologicals, CO, USA), mouse anti-Aβ (#10326, 1:200; IBL, Nagoya, Japan), mouse antiGFAP (#G3893, 1:250, Sigma), rat anti-C3 (#sc58926, 1:200; Santa Cruz Biotechnology, Inc., CA, USA), rabbit anti-BACE1 (#5606, 1:100, Cell Signaling, Inc., CA, USA), and |
